Nov 16, 2018
Biological safety cabinets are designed to protect the operator performing work on hazardous pathogens, using inflow velocity to prevent the pathogens from escaping from the work zone. About 95% of biosafety cabinets sold in the market is Class II Type A2 which also employ downflow that protect the product, by continuously bathing the work zone with laminar air flow. The inflow and front portion of the downflow creates V-shaped air curtain at the front opening that prevents biohazard material from escaping, and prevents outside contaminants from entering the work zone. The detailed airflow pattern is as shown below:
Figure 1: Cabinet Filtration System Diagram
From the airflow diagram above, the crucial elements for biosafety cabinet operation are the filters and blower. The blower pulls the air through the filter, which captures the contaminants and pathogens, and purifies the air. The selection of the filter determines the level of safety provided by the biosafety cabinet.
Figure 2: Particle Size Chart / Relative Size of Common Airborne Biohazards
The graphic shows an overview of pollutants from various industries and their corresponding particle sizes. The majority are not visible to the naked eyes and that such particles have the most negative health effects. Upon prolonged exposure, such pollutants may deposit in the lungs thus affecting normal respiratory functions. It should be noted that HEPA filter only cover about half of virus, but ULPA filter covers the entire size band of virus, giving the ULPA filter an edge in protecting the user and environment.
HEPA filter media is composed of a mat of randomly arranged non-synthetic borosilicate glass fibers, and taking a physical shape similar to a sheet of thick and fibrous paper. Particles passing these fibers are retained by physical cohesion, and not by electrostatic charge, that occurs in low end synthetic filter fiber. The downside of the synthetic fiber is that the particle retention is compromised once the electrostatic charge disappears, like when the airflow passing the media stops, and the retention efficiency decreases over time. When the airflow stops or over years of usage, the high end non-synthetic fiber will still retain the captured particles, using the three retention principles shown on the next table.
|1||Inertia||Large particles (about 0.5 to 5 micron) have too much inertia and hence deviate from the air stream around filter fibers, so they impact on the filter fiber and be retained by cohesion|
|2||Interception||Medium particles (about 0.1 to 0.5 micron) follow the air stream around filter fibers, however when they come in *side* contact with a filter fiber they are intercepted|
|3||Diffusion||Small particles (below 0.1 micron) oscillate according to Brownian motion due to bombardment by air molecules. When they touch the fiber, they are retained by cohesion.|
Table 1. Filter Methods of Collection Illustration
The sieving filtration principle was not included on the table above, because the common assumption that a HEPA filter primarily acts like a sieve is incorrect. The air space between HEPA filter fibers is much greater than 0.3 μm. Although it’s correct that very large particles with bigger diameter than the distance between fibers will be retained, HEPA filters are primarily designed to target much smaller particles.
The fiber density plays an important role in determining the filter efficiency. Increasing the fiber density leads to a filter type that is more efficient than HEPA, called ULPA (Ultra Low Penetration Air) filter.
The filter efficiency is also affected by the particle size. By observing the table above, the most particle size to be captured is the small to medium size particles, commonly referred as Most Penetrating Particle Size (MPPS). MPPS is the specific particle size that is not too large to be captured by the interception effect, and not too small to be captured by the diffusion effect.
Many contamination sensitive applications require “clean air”, which can be defined as a space where particle concentration in the air is controlled to an appropriate level. The most internationally recognized standard for clean air is ISO 14644, which defines the particles as solid or liquid objects between 0.1 to 5 micron, and the particle concentration as the number of individual particles per unit volume of air.
The particle size distribution is the cumulative distribution of particle concentration with respect to particle size, illustrated by the table below:
|ISO Class||Maximum number of particles allowed per 1 m3 of space|
|0.1 micron||0.2 micron||0.3 micron||0.5 micron||1 micron||5 micron|
Table 2.1: ISO Clean Air Standard
Typically clean air devices work by using fan(s) to move air through High Efficiency Particulate Air (HEPA) filter(s). The minimum cleanliness required for various applications are indicated below:
|3 or 4||Sensitive semiconductor or pharmaceutical work|
|5||Laminar flow cabinets, biological safety cabinets, pharmaceutical isolators|
|7||Clean room for pharmaceutical preparation, used with laminar flow or biosafety cabinets inside|
|8||Typical hospital environment|
|9||Typical office or labs without air filtering|
Table 2.2: ISO Class Application
HEPA and ULPA Filter Efficiency Classification
According to the American IEST-RP-CC001.3 guidelines, the HEPA filter has minimum typical efficiency of 99.99% at particle size of 0.3 microns. When combined with sufficient airflow and appropriate cabinet design, this filter can provide ISO Class 5 cleanliness required by laminar flow, biosafety cabinets, and isolators.
For cleaner environment requirement of ISO Class 4, 3, or better, ULPA filters can be used, due to its’ efficiency rating of 99.999% or better, at particle size between 0.1 to 0.2 microns, per IEST-RP-CC001.3 guidelines.
In Europe, filter performance classification is governed by EN 1822.1 standard. The minimum filter requirement for biosafety cabinet and isolators is H14, where the filters should have the efficiency of 99.995% or better at MPPS. The European EN 1822.1 standard does not mention the efficiency requirement at specific particle size, like the American IEST-RP-CC001.3.
|99.95% at MPPS||HEPA H13||Not allowed for BSC|
|Min requirement for BSC||HEPA||99.99% at 0.3 micron|
|99.995% at MPPS||HEPA H14||Min requirement for BSC|
|Advanced Safety BSC||ULPA||99.999% at 0.1-0.2 micron|
|99.9995% at MPPS||ULPA U15||Advanced Safety BSC|
Table 3. Summary of the difference between HEPA and ULPA Filters (US and European Standard)
MPPS is Most Penetrating Particle Size, which is the specific particle size that is most difficult to be captured by filter fibers, particle small enough to follow air stream around the fibres (avoid side interception) but has enough mass so the Brownian movement have minima l effect (avoid diffusion)
The European Standard EN 12469 for biosafety cabinet has a minimum requirement for 99.995% efficient filter, and anything less than that (including 99.99%) is not allowed for biosafety cabinets. Filter manufacturers following EN 1822 typically make the filters to have slightly better efficiency than this minimum requirement for safety buffer. Therefore, filters that are ordered to meet this 99.995% minimum efficiency has actual efficiency of 99.999% or better, which falls into ULPA filter category based on US IEST standard.
Based on the efficiency difference above, ULPA filter offers 10x better filtration that translates to being 10x safer than HEPA filter. At that efficiency, if the user releases 1 million spores of hazardous pathogens such as Tuberculosis, only 1 would escape through ULPA filter compared to 10 from HEPA filter. This can make a difference between staying healthy or getting infected. For simplicity, if we are using the US IEST definition of HEPA vs ULPA, then below is the summary of their difference:
|1 Million Spores||10 escape||1 escape|
|Work Zone||ISO 5 -4||ISO 3|
|Life||8 – 10 years||8 – 10 years|
|Cost to Replace||$ 400 - 600||$ 400 - 600|
Table 4: Summary of HEPA and ULPA Filter Difference (US IEST)
This additional level of efficiency comes at a slightly increased pressure drop of 5%, contrary to some beliefs that it is as high as 20 – 50%. To easily compensate for this minor pressure increase, the number of filter pleats or the filter width are slightly increased, to pack larger filter media, that lowers the pressure drop back to the same level as HEPA filter. This larger filter media also translates to longer filter life, because there is a larger area to capture the filters. By using this compensation method, ULPA filters have comparable life as HEPA filters at 8-10 years.
Figure 3. Filter Medium
The criteria to change the filter is when the blower is already running at maximum capacity, but unable to generate the required airflow anymore. Therefore, filter life is not just determined by filter pressure drop alone, but it’s actually determined by the combination of filter pressure drop, manufacturer’s airflow setpoint, cabinet airflow path design, motor-blower HP rating, impeller diameter, and impeller width. Therefore, another way to compensate for the slightly higher pressure drop in ULPA filter is by using slightly higher motor HP rating and optimizing the blower impeller size. By combining the latest DC-ECM motor with larger filter media and aerodynamic cabinet airflow path design, the cabinet energy consumption can be lowered.
Figure 4. Blower Motor
Contrary to some belief, the cost of ULPA filter with 99.999% efficiency is just about 5% more than HEPA filter with 99.99% efficiency, which is only about $20-$30 more, comparable to a lunch. If this is amortized to 10 years filter life, this becomes $2-$3 per year, comparable to 2 candy bars, which hardly make a difference in filter price, and completely outweigh the benefit of having 10x safety lab and cleaner work zone.
A good biosafety cabinet design optimizes the filtration efficiency, power consumption, airflow velocity, and filter life, to give the best combination of safety and operating cost. Some biosafety cabinet manufacturers may claim that their HEPA filters have longer life than ULPA, but after considering other design aspects, this claim no longer holds true. In fact, if their cabinet design is outdated, their power consumption is actually higher than modern biosafety cabinets equipped with ULPA filter. Moreover, this outdated design typically sacrifice the safety by lowering the downflow velocity that weakens the product protection and front air curtain. This effect is more pronounced in the real-world condition where the operators move their arms.
Figure 5.1: Operator Protection Test
Figure 5.2: Product Protection Test
Biosafety cabinets are validated by certification bodies such as NSF International, who perform microbiological testing. Operator protection test is performed by spraying spores from inside the cabinet, and sampling the air outside the front opening to quantify how many spores are captured. Product protection test is performed by spraying spores outside the cabinet and covering the entire work zone with agar plates to quantify how many spores managed to go inside. Both tests are conducted in static condition, where there are no arm movements, and the operator arms is only represented by a single stationary metal cylinder. This simplified test allows cabinets with weaker downflow velocity to pass.
However, in real world condition, the operator move both arms, causing dynamic airflow disturbance that can disrupt the front air curtain that prevents biohazard from escaping out and prevent outside contaminants to enter the work zone. This disturbance effect is amplified if the biosafety cabinet has larger sash opening, such as 10” and 12”, because the inflow underneath the sash is at the weakest, being far away from the front grille. Stronger downflow helps to maintain this air curtain despite the airflow disturbance created by the operator’s arm movement.
Another safety factor that can be compromised by outdated biosafety cabinet design is the light intensity. Such cabinets may have only single fluorescent tube, generating 90 ft-cd light intensity on the work zone. Meanwhile, a safer modern biosafety cabinet design has 130 ft-cd light intensity, to provide sufficient illumination for critical work, and prevent hazardous self-inoculation.
Figure 6: Optimum 130 ft-cd Light Intensity
Therefore, outdated biosafety cabinets that use a combination of HEPA filter, inefficient aerodynamics, weaker downflow velocity, weaker light intensity, and weaker motor-blower that runs at higher speed with clean filters, actually consumes more power and has lower motor reserve capacity to overcome filter pressure drop. To achieve the desired 8-10 years filter life, the downflow velocity and light intensity are lowered, that translates to riskier work environment than modern cabinets with ULPA filter and stronger & newer motor, that yields stronger downflow and brighter light intensity, while lowering power consumption.
Purchasing biosafety cabinets means purchasing a complete system, not just a box with HEPA / ULPA filter, so the filtration efficiency, downflow velocity, light intensity, and power consumption have to be compared, to differentiate between legacy versus modern cabinets that provides safer work environment and lower energy consumption at same filter life and same replacement cost.
Esco Micro Pte. Ltd.
21 Changi South Street 1 Singapore 486777
+65 65420833 | +65 65426920